Fig 1: High magnification images were shown of immunolabeling aquaporin (AQP) 2 and arginine vasopressin receptor (AVPR) 2 in normal canine kidney (a and b) and were compared to immunolabeling AQP2 and AVPR2 in chronic kidney disease (CKD) (c and d). The protein localization and expression were shown in red color. The immunolabeling revealed that AQP2 (c) and AVPR2 (d) expressions were lower in canines with CKD when compared to AQP2 (a) and AVPR2 (b) expression in normal kidney. CD=Collecting duct, LH=Thin segment Henle’s loop.
Fig 2: (a and b) Semi-quantitative analysis of aquaporin (AQP) 2 and arginine vasopressin receptor (AVPR) 2 immunostaining in canines with chronic kidney disease (CKD) and normal kidney (NORM). The Mann–Whitney U-test: Values are expressed as mean±SEM, with *p<0.001 significance level. Semi-quantitative analysis of CKD revealed a significant decrease in the percentage of protein expression in AQP2 and AVPR2.
Fig 3: Low and high magnification of histological and immunohistochemical localization of aquaporin (AQP) 2 and arginine vasopressin receptor (AVPR) 2 in the cortex and medulla. Negative control in the cortex (a) and medulla (b). Immunolocalization immunoperoxidase labeling of AQP2 in the cortex (c and e, red arrow) and medulla (d and f, blue arrow). Immunolocalization immunoperoxidase labeling of AVPR2 in the cortex (g, red arrow) and medulla (h, blue arrow). The proteins’ localization and expression were shown in brown. AQP2 was found in the basolateral and apical membranes of the principal cells, whereas AVPR2 was found in the basolateral membrane of the principal cells in the renal cortex and the renal medulla. CD=Collecting duct, DCT=Distal convoluted tubule, G=Glomerulus, IC=Intercalated cell, LH=Thin segment Henle’s loop, PC=Principal cell, PCT=Proximal convoluted tubule, RBC=Red blood cell, TAL=Thick ascending Henle’s loop, TDL=Thick descending Henle’s loop.
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